Alan S. L. Yu, M.B., B.Chir.
Associate Professor of Medicine
1983-1986 University
of Cambridge, England, B.A. (Honors, Class I), Medical Sciences
1986-1989 University
of Cambridge, England, M.B., B.Chir. (Distinction), Medicine
1990-1993 Research Fellow in Nephrology, Brigham & Women's Hospital and Harvard Medical School (Mentors: Jonathan Lytton, Ph.D., Steven C. Hebert, M.D.)
1992-1993 Intern in Internal Medicine, Physician-Scientist Track, Brigham & Women's Hospital
1993-1995 Resident in Internal Medicine, Physician-Scientist Track, Brigham & Women's Hospital
1995-1996 Clinical Fellow in Nephrology, Brigham & Women's Hospital
1995-2000 Instructor
in Medicine, Harvard Medical School
1996-2002 Associate
Physician, Brigham & Women's Hospital
2000-2002 Assistant
Professor of Medicine, Harvard Medical School
2002-2005 Assistant Professor of Medicine, Physiology and Biophysics, USC Keck School of Medicine
2005- Associate Professor of Medicine, Physiology and Biophysics, USC Keck School of Medicine
1983 Entrance
Scholarship, Cambridge University
1984 Junior
Scholarship, Cambridge University
1987 Charles
Davis Prize in Medicine, University of London
1989 Harold
Boldero Prize in Medicine, Lyell Prize in Surgery, Hobbiger Prize in Clinical
Pharmacology, Hetley Scholarship in Clinical Sciences, & William Henry Rean
Graduation Prize, University of London
1989 Distinction
in Medicine, Cambridge University
1990 M.A.
(Honorary), Cambridge University
1991 Research
Fellowship, National Kidney Foundation
1996 Joseph
E. Murray Research Award, National Kidney Foundation
1998 Carl
W. Gottschalk Research Scholar Award, American Society of Nephrology
2000 Daniel
D. Federman Outstanding Clinical Educator Award, Harvard Medical School
2000-2003 Section
Editor, Current Opinion in Nephrology and Hypertension
2000-Present External
reviewer, Wellcome Trust Programme Grants (UK)
2001-Present External
Referee Panel, National Kidney Research Fund (UK)
2003 American
Heart Association, Western Review Consortium Committee
2003-2006 Editorial
Board, Journal of the American Society of Nephrology (JASN)
2003-Present Editorial
Board, American Journal of Physiology: Renal Physiology
2003 Ad
hoc member, NIH General Medicine B Study Section
2004 Ad
hoc member, VA Nephrology Merit Review Subcommittee
2006-Present Editorial Board, Journal of Biological Chemistry
2007-Present Regular member, NIH Cellular and Molecular Biology of the Kidney Study Section
C. Peer-Reviewed Publications
1. Heilig CW,
Brenner RM, Yu ASL,
Kone BC, Gullans SR. Modulation of osmolytes in MDCK cells by solutes,
inhibitors and vasopressin. Am. J.
Physiol. (1990) 259 : F653-659.
2. Yu ASL, Hebert SC, Brenner BM, Lytton J.
Molecular characterization and nephron distribution of the pore-forming subunit
of a family of renal Ca2+ channels. Proc. Natl. Acad. Sci. USA.
(1992) 89: 10494-10498.
3. Yu ASL, Hebert SC, Lee S-L, Brenner BM,
Lytton J. Identification and localization of the renal Na+-Ca2+ exchanger by the
polymerase chain reaction. Am. J. Physiol. (1992) 263: F680-F685.
4. Lee S-L, Yu
ASL, Lytton J.
Tissue specific expression of Na+-Ca2+ exchanger
isoforms. J. Biol. Chem. (1994) 269: 14849-14852.
5. Yu ASL, Boim M, Hebert SC, Castellano A,
Perez-Reyes E, Lytton J. Molecular characterization of renal calcium channel b subunit transcripts. Am. J. Physiol. (1995) 268: F525-F531.
6. Van Baal J, Yu
ASL, Hartog A,
Fransen JAM, Willems PHGM, Lytton J, Bindels RJM. Localization and regulation
by vitamin D of calcium transport proteins in rabbit cortical collecting
system. Am. J. Physiol. (1996) 271: F985-F993.
7. Yu ASL: Atypical electrocardiographic changes in severe hyperkalemia. Am J Cardiol 77:906-908, 1996.
8. Yu ASL, Levy E: Paradoxical cerebral air embolism from a hemodialysis catheter. Am J Kidney Dis 29:453-455, 1997.
9. Barry ELR, Gesek
FA, Yu ASL,
Lytton J, Friedman PA. Distinct calcium channel isoforms mediate parathyroid
hormone and chlorothiazide-stimulated calcium entry in transporting epithelial
cells. J. Memb. Biol. (1998) 161: 55-64.
10. Luyckx VA, Goda FO, Mount DB,
Nishio T, Hall A, Hebert SC, Hammond TG, Yu ASL. Intrarenal and subcellular
localization of rat CLC5. Am. J. Physiol. (1998) 275: F761-F769.
11. Luyckx VA, Leclercq B, Dowland L, Yu
ASL. Diet-dependent
hypercalciuria in transgenic mice with reduced CLC5 chloride channel
expression. Proc. Natl. Acad. Sci. USA. (1999) 96, 12174-12179.
12. Pavlova A, Sakurai H, Leclercq B,
Beier DR, Yu ASL, Nigam SK.
Developmentally regulated expression of the organic ion transporters, NKT/mOAT,
OCT-1, NLT and Roct during murine embryogenesis. Am. J. Physiol. Renal Physiol.
(2000) 278: F635-F643.
13. Wills NK, Weng T, Mo L, Hellmich
HL, Yu ASL, Wang T, Bucheit
S, Godley BF. Chloride channel expression in cultured human fetal RPE cells:
Response to oxidative stress. Invest. Ophthalm. Vis. Sci. (2000) 41:4247-4255.
14. Dowland LK, Luyckx VA, Enck AH,
Leclercq B, Yu ASL. Molecular
cloning and characterization of an intracellular chloride channel in the
proximal tubule cell line, LLC-PK1. J. Biol. Chem. (2000) 275:
37765-37773.
15. Weng TX, Mo L, Hellmich HL, Yu
ASL, Wang T, Wills NK.
Expression and regulation of ClC-5 chloride channels: Effects of antisense and
oxidants. Am J Physiol Cell Physiol (2001) 280: C1511-20.
16. Enck AH, Berger U, Yu ASL. Claudin-2 is selectively expressed in the proximal nephron in mouse kidney. Am. J. Physiol. Renal Physiol. (2001) 281: F966-74.
17. TX Weng, BF Godley, GF Jin, NJ
Mangini, BG Kennedy, Yu ASL,
NK Wills. Chloride channels expressed in human adult retina. Am. J. Physiol.
Cell. Physiol. (2002) 283:C839-C849.
18. Isnard-Bagnis C, Da Silva N,
Beaulieu V, Yu ASL, Brown D,
Breton S. Detection of the chloride channels ClC-3 and ClC-5 in epithelial
cells of the epididymis by immunofluorescence and RT-PCR after laser capture
microdissection. Am. J. Physiol. Cell. Physiol.(2003) 284:C220-C232.
19. Yu ASL, Enck AH, Lencer WI, Schneeberger EE. Functional expression of claudin-8 reveals a role in the paracellular cation barrier. J. Biol. Chem. (2003) 278:17350 -17359.
20. Li WY, Huey CL, Yu ASL. Expression of claudins 7 and 8 along the mouse
nephron. Am. J. Physiol. Renal Physiol. (2004) 286:F1063-F1071.
21. Huey CL, Riepe FG, Sippell WG, Yu
ASL. Genetic heterogeneity in
autosomal dominant pseudohypoaldosteronism type I: Exclusion of claudin-8 as a
candidate gene. Am J Nephrol. (2004) 24:483-487.
22. Yu ASL, McCarthy KM, Francis SA, McCormack JM, Lynch RD, Schneeberger EE. Knockdown of occludin expression leads to diverse phenotypic alterations in epithelial cells. Am J Physiol Cell Physiol (2005) 288:C1231-C1241.
23. Angelow S, Kim KJ, Yu ASL. Claudin-8 modulates paracellular permeability to acidic and basic ions in MDCK II cells. J Physiol 571:15-26, 2006.
24. Van Itallie CM, Rogan S, Yu ASL, Seminario L, Holmes J, Anderson JM. Two splice variants of claudin-10 in the kidney create paracellular pores with different ion selectivities. Am J Physiol Renal Physiol (2006) 291:F1288-1299.
25. Angelow S, Schneeberger EE, Yu ASL. Claudin-8 expression in renal epithelial cells augments the paracellular barrier by replacing endogenous claudin-2. J Memb Biol 215(2-3):147-59, 2007.
25. Angelow S, Schneeberger EE, Yu ASL: Claudin-8 expression in renal epithelial cells augments the paracellular barrier by replacing endogenous claudin-2. J Memb Biol 215(2-3):147-59, 2007.
26. Angelow S, El-Husseini R, Kanzawa SA, Yu ASL: Renal localization and function of the tight junction protein, claudin-19. Am J Physiol Renal Physiol 293(1):F166-77, 2007.
27. S Selvam, PB Thomas, Hovik J Gukasyan, ASL Yu, D Stevenson, MD Trousdale, AK Mircheff, JE Schechter, SC Yiu. Transepithelial bioelectrical properties of rabbit acinar cell monolayers on polyester membrane scaffolds. Am J Physiol Cell Physiol 293:C1412-9, 2007.
28. Scheiermann C, Meda P, Aurrand-Lions M, Madani R, Yiangou Y, Coffey P, Salt TE, Ducrest-Gay D, Caille D, Howell O, Reynolds R, Lobrinus A, Adams RH, Yu ASL, Anand P, Imhof BA, Nourshargh S. Expression and function of junctional adhesion molecule (JAM)-C in myelinated peripheral nerves. Science 318:1472-5, 2007.
29. Hsu CT, Wang ZJ, Yu ASL, Gould RG, Fu Y, Joe BN, Qayyum A, Breiman RS, Coakley FV, Yeh BM: Physiology of renal medullary tip hyperattenuation on unenhanced CT: Urinary specific gravity and the sodium chloride concentration gradient. Radiology 247(1):147-53, 2008.
30. Sabath E, Negoro H, Beaudry S, Paniagua M, Angelow S, Shah J, Grammatikakis N, Yu ASL, Denker BM. Ga12 regulates both maintenance and assembly of tight junctions through Src and the Hsp90 heat shock protein complex. J Cell Sci 121:814-24, 2008.
31. Yu ASL, Kanzawa SA, Usorov A, Lantinga-van Leeuwen IS, Peters DJM. Tight junction composition is altered in the epithelium of polycystic kidneys. J Pathol (in press).
D. Invited Reviews
1. Brenner BM, Yu ASL: Uremic syndrome revisited: A pathogenetic role for retained endogenous inhibitors of nitric oxide synthesis. Curr Opin Nephrol Hypertens 1:3-7, 1992.
2. Yu ASL: Calcium channels. Curr Opin Nephrol Hypertens 3:497-503, 1994.
3. Yu ASL:
Identification and localization of calcium channel a1 and b subunit isoforms in the kidney. Kidney Int 48:1097-1101, 1995.
4. Yu ASL: Paracellular solute transport: more than just a leak? Curr Opin Nephrol Hypertens 9:513-515, 2000.
5. Yu ASL: Role of ClC-5 in the pathogenesis of hypercalciuria: recent insights from transgenic mouse models. Curr Opin Nephrol Hypertens 10:415-420, 2001.
6. Yu ASL: Evolving concepts in epithelial magnesium transport. Curr Opin Nephrol Hypertens 10:649-653, 2001.
7. Yu ASL: Elucidating the structural basis of membrane transport protein function: Light at the end of the channel? Curr Opin Nephrol Hypertens 11:523-526, 2002.
8. Yu ASL: WNK signalling in the distal tubule: An inhibitory cascade regulating salt transport. Am J Physiol Renal Physiol 290:F617-618, 2006.
9. Angelow S, Yu ASL: Claudins and paracellular transport; an update. Curr Opin Nephrol Hypertens, 16(5):459-464, 2007.
10. Angelow S, Ahlstrom R, Yu ASL: Biology of claudins. Am J Physiol Renal Physiol in press.
11. Yu ASL: Molecular basis for cation selectivity in claudin-2-based pores. Ann NY Acad Sci in press.
12. Angelow S, Yu ASL: Cysteine mutagenesis to study the structure of claudin-2 paracellular pores. Ann NY Acad Sci in press.
13. Günzel D, Fromm M, Yu ASL. Function and regulation of claudins in the thick ascending limb of Henle. Pflüger's Arch Eur J Physiol in press.
E. Book Chapters
1. Yu ASL: Calcium
channels. In: Molecular Nephrology: Kidney Function in Health and Disease (Bonventre JV, Schlondorff D [editors]). Marcel Dekker, New York, NY, pp427-438, 1995.
2. Yu ASL: Disturbances of magnesium metabolism. In: Brenner and Rector's The Kidney (6th edition; Brenner BM [editor]). Saunders, Philadelphia, PA, pp1055-1070, 2000.
3. Yu ASL, Brenner BM: Tubulointerstitial diseases of the kidney. In: Harrisons Principles of Internal Medicine (15th edition; Braunwald E, et al [editors]). McGraw-Hill, New York, NY, pp1606-1610, 2001.
4. Smirnakis KV, Yu
ASL: Diuretics in acute renal failure. In: Therapy of Nephrology and Hypertension
(2nd edition; Wilcox C, Brady HR [editors]). Saunders, Philadelphia, PA,
pp33-37, 2003.
6. Yu ASL: Renal
transport of calcium, magnesium and phosphate. In: Brenner and Rector's The
Kidney (7th edition; Brenner BM [editor]). Saunders, Philadelphia, PA, Vol. I.,
pp535-571, 2004.
7. Pollak M, Yu ASL:
Clinical disturbances of calcium, magnesium and phosphate metabolism. In:
Brenner and Rector's The Kidney (7th edition; Brenner BM [editor]). Saunders,
Philadelphia, PA, pp 1041-1076, 2004.
8. Yu ASL, Brenner BM: Tubulointerstitial diseases of the kidney. In: Harrisons Principles of Internal Medicine (16th edition; Kasper DL, et al [editors]). McGraw-Hill, New York, NY, pp 1702-1706, 2005.
9. Yu ASL, Denker B: Practice Examination. In: Educational Review Manual in Nephrology (1st edition; Singh A [editor]). Castle Connolly, New York, NY. In press.
10. Yu ASL: Disorders of magnesium and phosphate. In: Cecil Textbook of Medicine (23rd edition; Goldman L, Ausiello D [editors]). Saunders, Philadelphia, PA, pp 859-862, 2007.
11. Yu ASL, Brenner BM: Tubulointerstitial diseases of the kidney. In: Harrisons Principles of Internal Medicine (17th edition; Loscalzo J, et al [editors]). McGraw-Hill, New York, NY. In press.
12. Mount D, Yu ASL: Renal transport of inorganic solutes. In: Brenner and Rector's The Kidney (8th edition; Brenner BM [editor]). Saunders, Philadelphia, PA, pp 156-213, 2007.
13. Pollak MR, Yu ASL, Taylor EN: Disorders of Calcium, Magnesium, and Phosphate Balance. In: Brenner and Rector's The Kidney (8th edition; Brenner BM [editor]). Saunders, Philadelphia, PA, 588-612, 2007.
14. Nadim M, Yu ASL: Diuretics in acute renal failure. In: Therapy of Nephrology and Hypertension (3rd edition; Wilcox C, Berl T, Himmelfarb J, Murphy B, Salant D, Yu ASL [editors]). Saunders, Philadelphia, PA.
F. Books
1. Wilcox C, Berl T, Himmelfarb J, Murphy B, Salant D, Yu ASL (editors). Therapy of Nephrology and Hypertension, 3rd edition. Saunders, Philadelphia, PA. In press.
G. Research Support
ACTIVE
RO1 DK62283-05 (PI: Yu) 7/1/03-6/30/11
NIH-NIDDK
Expression,
function and regulation of renal claudins
Grant-In-Aid (PI: Yu)
PKD Foundation Research 2/1/07-1/31/09
Role of claudin-7 in chloride and fluid secretion in polycystic kidney disease
COMPLETED
Pilot
Grant (PI: Yu) 3/1/03-2/28/04
USC
NIDDK Research Center for Liver Diseases
Intestinal
paracellular barrier function
Extramural
Grant (PI: Yu) 10/01/01-9/30/04
Satellite
Research
The
Molecular Basis of Paracellular Transport in the Renal Tubule
Research
Grant (PI: Yu) 7/01/02-6/30/03
Wright
Foundation
Functional
Properties of a Putative Distal Nephron Paracellular Channel