Alan S. L. Yu, M.B., B.Chir.
Associate Professor of Medicine
1983-1986 University
of Cambridge, England, B.A. (Honors, Class I), Medical Sciences
1986-1989 University
of Cambridge, England, M.B., B.Chir. (Distinction), Medicine
1990-1993 Research Fellow in Nephrology, Brigham & Women's Hospital and Harvard Medical School (Mentors: Jonathan Lytton, Ph.D., Steven C. Hebert, M.D.)
1992-1995 Residency in Internal Medicine, Physician-Scientist Track, Brigham & Women's Hospital
1995-1996 Clinical Fellow in Nephrology, Brigham & Women's Hospital
1995-2000 Instructor
in Medicine, Harvard Medical School
2000-2002 Assistant
Professor of Medicine, Harvard Medical School
2002-2005 Assistant Professor of Medicine, Physiology and Biophysics, USC Keck School of Medicine
2005- Associate Professor of Medicine, Physiology and Biophysics, USC Keck School of Medicine
2003-2006 Editorial Board, Journal of the American Society of Nephrology
2003-Present Editorial
Board, American Journal of Physiology: Renal Physiology
2006-Present Editorial Board, Journal of Biological Chemistry
2007-Present Regular member, NIH Cellular and Molecular Biology of the Kidney Study Section
Selected Peer-Reviewed Publications
Yu ASL, Hebert SC, Brenner BM, Lytton J. Molecular characterization and nephron distribution of the pore-forming subunit of a family of renal Ca2+ channels. Proc. Natl. Acad. Sci. USA. (1992) 89: 10494-10498.
Luyckx VA, Leclercq B, Dowland L, Yu ASL. Diet-dependent hypercalciuria in transgenic mice with reduced CLC5 chloride channel expression. Proc. Natl. Acad. Sci. USA. (1999) 96, 12174-12179.
Dowland LK, Luyckx VA, Enck AH, Leclercq B, Yu ASL. Molecular cloning and characterization of an intracellular chloride channel in the proximal tubule cell line, LLC-PK1. J. Biol. Chem. (2000) 275: 37765-37773.
Yu ASL, Enck AH, Lencer WI, Schneeberger EE. Functional expression of claudin-8 reveals a role in the paracellular cation barrier. J. Biol. Chem. (2003) 278:17350 -17359.
Yu ASL, McCarthy KM, Francis SA, McCormack JM, Lynch RD, Schneeberger EE. Knockdown of occludin expression leads to diverse phenotypic alterations in epithelial cells. Am J Physiol Cell Physiol (2005) 288:C1231-C1241.
Angelow S, Kim KJ, Yu ASL. Claudin-8 modulates paracellular permeability to acidic and basic ions in MDCK II cells. J Physiol 571:15-26, 2006.
Angelow S, Schneeberger EE, Yu ASL. Claudin-8 expression in renal epithelial cells augments the paracellular barrier by replacing endogenous claudin-2. J Memb Biol 215(2-3):147-59, 2007.
Angelow S, El-Husseini R, Kanzawa SA, Yu ASL: Renal localization and function of the tight junction protein, claudin-19. Am J Physiol Renal Physiol 293(1):F166-77, 2007.
Scheiermann C, Meda P, Aurrand-Lions M, Madani R, Yiangou Y, Coffey P, Salt TE, Ducrest-Gay D, Caille D, Howell O, Reynolds R, Lobrinus A, Adams RH, Yu ASL, Anand P, Imhof BA, Nourshargh S. Expression and function of junctional adhesion molecule (JAM)-C in myelinated peripheral nerves. Science 318:1472-5, 2007.
Sabath E, Negoro H, Beaudry S, Paniagua M, Angelow S, Shah J, Grammatikakis N, Yu ASL, Denker BM. Ga12 regulates both maintenance and assembly of tight junctions through Src and the Hsp90 heat shock protein complex. J Cell Sci 121:814-24, 2008.
Yu ASL, Kanzawa SA, Usorov A, Lantinga-van Leeuwen IS, Peters DJM. Tight junction composition is altered in the epithelium of polycystic kidneys. J Pathol 216(1):120-128, 2008.
Yu ASL, Cheng, MH, Angelow S, Günzel D, Kanzawa SA, Schneeberger EE, Fromm M, Coalson RD. Molecular basis for cation selectivity in claudin-2-based paracellular pores: Identification of an electrostatic interaction site. J Gen Physiol, 133: 111-127, 2009.
Ahlstrom R, Yu ASL. Characterization of the kinase activity of a WNK4 protein complex. Am J Physiol Renal Physiol, 297(3):F685-92, 2009.
Angelow S, Yu ASL. Structure-function studies of claudin extracellular domains by cysteine-scanning mutagenesis. J Biol Chem, 284(42):29205-17, 2009.
Recent Invited Reviews
Angelow S, Yu ASL: Claudins and paracellular transport; an update. Curr Opin Nephrol Hypertens, 16(5):459-464, 2007.
Angelow S, Ahlstrom R, Yu ASL: Biology of claudins. Am J Physiol Renal Physiol 295(4):F867-76, 2008.
Yu ASL: Molecular basis for cation selectivity in claudin-2-based pores. Ann NY Acad Sci 1165: 53–57, 2009.
Angelow S, Yu ASL: Cysteine mutagenesis to study the structure of claudin-2 paracellular pores. Ann NY Acad Sci 1165: 143-147, 2009.
Günzel D, Yu ASL. Function and regulation of claudins in the thick ascending limb of Henle. Pflüger's Arch Eur J Physiol 458, 77-88, 2009.
Recent Book Chapters
Yu ASL: Disorders of magnesium and phosphate. In: Cecil Textbook of Medicine (23rd edition; Goldman L, Ausiello D [editors]). Saunders, Philadelphia, PA, pp 859-862, 2007.
Yu ASL, Brenner BM: Tubulointerstitial diseases of the kidney. In: Harrisons Principles of Internal Medicine (17th edition; Loscalzo J, et al [editors]). McGraw-Hill, New York, NY. In press.
Mount D, Yu ASL: Renal transport of inorganic solutes. In: Brenner and Rector's The Kidney (8th edition; Brenner BM [editor]). Saunders, Philadelphia, PA, pp 156-213, 2007.
Pollak MR, Yu ASL, Taylor EN: Disorders of Calcium, Magnesium, and Phosphate Balance. In: Brenner and Rector's The Kidney (8th edition; Brenner BM [editor]). Saunders, Philadelphia, PA, 588-612, 2007.
Nadim M, Yu ASL: Diuretics in acute renal failure. In: Therapy of Nephrology and Hypertension (3rd edition; Wilcox C, Berl T, Himmelfarb J, Murphy B, Salant D, Yu ASL [editors]). Saunders, Philadelphia, PA.
Edited Books
Wilcox C, Berl T, Himmelfarb J, Murphy B, Salant D, Yu ASL (editors). Therapy of Nephrology and Hypertension, 3rd edition. Saunders, Philadelphia, PA. In press.
Research Support
ACTIVE
RO1 DK62283-05 (PI: Yu) 7/1/03-6/30/11
NIH-NIDDK
Expression,
function and regulation of renal claudins
Grant-In-Aid (PI: Yu)
PKD Foundation Research 2/1/07-1/31/09
Role of claudin-7 in chloride and fluid secretion in polycystic kidney disease