Laboratory of Vertebrate Brain Mapping
University of Southern California
Keck School of Medicine
Dept. of Cell and Neurobiology
1333 San Pablo St., BMT 403, MC 9112
Los Angeles, CA 90033
RESEARCH INTERESTS: A central dilemma in functional neuroimaging of animal behaviors has been the fact that conventional neuroimaging protocols using positron emission tomography (PET), single photon emission computer tomography (SPECT) and functional magnetic resonance imaging (fMRI) generally rely on immobilization of the subject, which extinguishes all but the simplest activity and introduces the additional variables of restraint stress. The result is that brain function of core mammalian behaviors such as aggression, mating, foraging and social interaction all requiring locomotion -- remains poorly understood. In our previous work, we have designed and validated the use of an implantable, miniature, infusion pump (MIP) for the administration of radiotracers in freely moving animals. Current projects in my lab focus on: (a) applications of the MIP in rats that allow functional brain mapping of complex behaviors using autoradiography (http://www.nibib.nih.gov/publicPage.cfm?pageid=657) , (b) study of the MIP in combination with novel radiotracers to allow an expanded use of PET for functional neuroimaging applications in freely-moving animals, (c) the miniaturization of the MIP for use in mice.
Complementary to the focus on functional brain mapping, is our work concerning the characterization of the neurobiology, behavior and physiologic function of specific animal models of human behavior and mental illness. Past work has included two different cholinergic deafferentation model of dementia in rats, mice deficient in monoamine oxidase which show varying amounts of aggression and anxiety depending on which of two of the isoform of the enzyme is lacking. Current work focuses on a rat model of Parkinson's disease, and the conditioned fear model of anxiety
A final focus is in the area of technology development. An area of active work is the development of methods for the analysis of brain mapping data obtained from rodents. Another concerns a method for measuring cardiac output by dye dilution and transcutaneous fluorescence - a technique that we are developing for use in humans, as well as transgenic mice. Interdisciplinary research collaborations are ongoing with the USC Dept of Bioengineering (Dr. J-M Maarek), the USC School of Pharmacy (Drs. JC Shih, and K Chen), the USC/Alfred E. Mann Institute, the UCLA Dept. of Physiology (Dr. OU Scremin), and the UCLA Dept. of Anesthesia (Dr. E Rubinstein).
Holschneider DP, Scremin OU, Chialvo D, Chen K, Shih JC: Heart Rate Dynamics in Monoamine Oxidase -A and -B Deficient Mice. American Journal of Physiology 282(5):H1751-9, 2002.
Holschneider DP, Scremin OU, Roos KP, Chialvo D, Chen K, Shih JC : Increased Baroreceptor Response in Mice Deficient in Monoamine Oxidase A and B. American Journal of Physiology 282:H964-H972, 2002.
Holschneider DP, Scremin OU, Huynh L, Chen K, Seif I, Shih JC : Regional Cerebral Cortical Activation In MAO-A Deficient Mice: Differential Effects Of Chronic Versus Acute Elevations In Serotonin And Norepinephrine. Neuroscience 101(4):869-877, 2000.
Holschneider DP, Scremin OU, Chen K, Shih JC : Lack of Protection of Monoamine Oxidase B-Deficient Mice from Age-related Spatial Learning Deficits in the Morris Water Maze. Life Sciences 65(17):1757-1763, 1999.
Scremin OU, Holschneider DP, Chen K, Li MG, Shih JC : Cerebral Cortical Blood Flow Maps are Reorganized in MAOB-deficient Mice. Brain Research 824:36-44, 1999.
Holschneider DP,Scremin OU, Huynh L, Chen K, Shih JC : Lack of Protection from Ischemic Injury of Monoamine Oxidase B-deficient Mice Following Middle Cerebral Artery Occlusion. Neuroscience Letters 259:3:161-164, 1999.
Investigations in Brain Electrical Function
Holschneider DP, Leuchter AF : Attenuation of Brain High Frequency Response After Thiopental in Subjects with Early Alzheimer's Dementia. Psychopharmacology 149(1):6-11, 2000.
Holschneider DP, Leuchter AF : Clinical Neurophysiology Using Electroencephalography in Geriatric Psychiatry: Neurobiological Implications and Clinical Utility. Journal of Geriatric Psychiatry and Neurology 12(3):150-164, 1999.
Holschneider DP, Waite J, Leuchter AF, Walton NY, Scremin OU : Changes in Electrocortical Power and Coherence in Response to the Cholinergic Toxin 192IgG-Saporin. Experimental Brain Research 126(2):270-280, 1999.
Holschneider DP, Leuchter AF, Scremin OU, Treiman DM, Walton NY : Effects of Cholinergic Deafferentation and NGF on Brain Electrical Coherence. Brain Research Bulletin 45:531-541, 1998.
Holschneider DP, Leuchter AF, Walton NY, Scremin OU, Treiman, DM : Changes in Cortical EEG and Cholinergic Function in Response to NGF in Rats with Nucleus Basalis Lesions. Brain Research 765:228-237, 1997.
Holschneider DP, Leuchter AF, Uijtdehaage SHJ, Abrams M, Rosenberg-Thompson S : Loss of High Frequency EEG Response to Thiopental in Alzheimer's Dementia. Neuropsychopharmacology 20:1-7, 1997.
Holschneider DP, Leuchter AF : Beta Activity in the Elderly: The Effects of Age, Gender, and Brain Disease. Brain Topography 8(2):169-180, 1995.
Studies in Cerebral Blood Flow
Holschneider DP, Yang J, Sadler TS, Nguyen PT, Givrad TK, Maarek JM : Mapping Cerebral Blood Flow Changes During Auditory Conditioned Fear in the Nontethered, Nonrestrained Rat. Neuroimage 2005, in press.
Holschneider DP, Maarek J-M I, Yang J, Harimoto J, Scremin OU : Activation of Cerebral Cortex During Acoustic Challenge or Acute Foot-shock in Freely Moving, Non-tethered Rats. Neuroscience Letters 354(1):74-78, 2004.
Holschneider DP, Maarek J-M I, Yang J, Harimoto J, Scremin OU : Functional Brain Mapping In Freely Moving, Nontethered Rats During Treadmill Walking. Journal of Cerebral Blood Flow and Metabolism 23(8):925-932, 2003.
Holschneider DP, Maarek J, Harimoto J, Yang, J, Scremin OU : An Implantable Bolus Infusion Pump For Use In Freely Moving, Nontethered Rats. American Journal of Physiology 283(4):H1713-19, 2002.
Waite J, Holschneider DP, Scremin OU : Selective Immunotoxin-Induced Cholinergic Deafferentation Alters Blood Flow Distribution in the Cerebral Cortex. Brain Research 818:1-11, 1999.
Holschneider DP, Scremin OU : Effect of Ovariectomy on Cerebral Blood Flow in Rats. Neuroendocrinology 67:260-268, 1998.
Holschneider DP, Maarek J-M I : Mapping Brain Function in Freely-Moving Subjects, Neuroscience and Biobehavioral Reviews, 28(5):449-461, 2004.
Yang J, Maarek JM, Holschneider DP : In Vivo Quantitative Assessment of Catheter Patency in Rats. Lab Animals 39(3):259-268, 2005.
Nguyen PT, Holschneider DP, Maarek J-MI, Yang J, Mandelkern MA : Statistical Parametric Mapping Applied to an Autoradiographic Study of Cerebral Activation During Treadmill Walking in Rats. Neuroimage 23:252-259, 2004.
Holschneider DP, Maarek J, Harimoto J, Yang J, Scremin OU : An Implantable Bolus Infusion Pump For Use In Freely Moving, Nontethered Rats. American Journal of Physiology 283(4):H1713-19, 2002.
Maarek J-M I, Holschneider DP, Scremin OU, Harimoto J, Yang J, Rubinstein E : Measurement of Cardiac Output with Indocyanine Green Transcutaneous Fluorescence Dilution Technique. Anesthesiology 100(6):1476-83, 2004.
Maarek J-M I, Holschneider DP, Yang J, Pniak SN, Yang J, Rubinstein E E : Transcutaneous fluorescence dilution cardiac output and circulating blood volume during hemorrhagic hypovolemia., Anesthesiology 102(4):774-82, 2005.