John Zhong , Ph.D.Assistant Professor, Pathology
DegreesUniversity of Southern California, Ph.D.,
University of Southern California, M.Sc.,
California State University, San Jose, B.Sc., 1997
Maltezos,G., Gomez, A., Zhong, J.F.,
Gomez, F.A. and Scherer, A.:Microfluidic polymerase chain
reaction. Appl. Phys.
Lett. 93, 243901, 2008
My research interests focus on developing
the knowledge base of gene regulation required to
support manipulation of stem cell differentiation
and self-renewal. Our research involves
the use of microfluidic processors for
simultaneous profiling of multiple single-cells.
In contrast to other investigators who study the
lysate of a cell population, we study the
molecular profiles of single-cells at continuous
developmental stages to investigate gene
Cell fate is determined by the gene-gene
interactions within a cell after receiving
environmental signals. Analyzing single-cells at
continuous developmental stages reveals the
stepwise gene-gene interaction during cell
development. A cell population often contains
cells at various developmental stages because of
the heterogeneity and asynchrony among cells.
Studying the lysate of a cell population can only
obtain population-averaging data which are not
informative enough to understand how gene-gene
interactions determine cell fates. Therefore,
studying gene regulation at the single-cell level
is necessary and effective
Zhong, J.F., Feng, Y., Taylor, C.R. Microfluidic Devices
for Investigating Stem Cell Gene Regulation via Single-cell
Analysis, Curr. Med.
Chem., 15 (28), 2897-2900, 2008
Zhong J.F, Chen Y, Marcus J.S, Scherer A, Quake S.R, Taylor
C.R and Weiner L.P: A Microfluidic Processor for Gene
Expression Profiling of Single Human Embryonic Stem Cells.
Lab-on-a-chip 7:68-74, 2008.
Zhong J.F, Song Y, Du J,
Gamache C, Burke K, Lund BT and Weiner LP: Gene Regulation
Networks Related to Neural Differentiation of hESC. Gene
Expression 14(1): 23-34, 2007.
Zhong J.F and Weiner
L.P: Role of Fetal Stem Cells in Tissues Regeneration during
Pregnancy. Gene Regulation and Systems Biology. Gene
Regulation and Systems Biology 1:111–115, 2007
J.F, Weiner L.P, Burke K, and Taylor C.R: Viral RNA Extraction
for In-the-Field Analysis. Journal of Virological Methods
144(1-2): 98-102, 2007
Zhong J.F, Maltezos G, Sheriff
Z, Burke K, Scherer A, Taylor C.R and Anderson W.F:
Microfluidic Systems for Studying Cell Migration Regulation.
Letters in Drug Design & Discovery 3: 636-639, 2006
Kartalov EP, Zhong J.F, Scherer A, Quake S.R, Taylor
C.R, and Anderson W.F: High-Throughput Multi-Antigen
Microfluidic Fluorescence Immunoassays. Biotechniques