Thomas D. Coates, M.D.

Punam Malik, M.D.

Martine Torres, Ph.D.

Eugenio De Hostos, Ph.D.

Four-Dimensional Imaging of mature human neutrophils derived from human CD34+ progenitors transfected with GFP-Actin.

A Z-series at 1 micron intervals of a  PMN moving on a surface was acquired every 10 seconds.

The time series here is  formed from 1 micron sections at the plane of contact of the PMN with the surface taken from Z-series stacks acquired at 10 second intervals and restored by exhaustive photon reassigment.

The extension of a single lamellipod can be seen as this PMN starts to move.  A dense cortical rim of actin is present in the back of cell and seems to squeeze the cytoplasm toward the extending lamella. 

(Web posting courtesy of Mr. David L. Loor)

tom1.avi - 101kb

tom1.mov - 120kb (click here if you use Netscape)

tom1.avi (102914 bytes) mont3.gif (158782 bytes)